對照（化蛹率90.00%、羽化率86.67%）。
關鍵詞 鉤枝藤；生物堿；斜紋夜蛾；毒殺活性；抑制生長發育
中圖分類號 S481.1 文獻標識碼 A
Insecticidal Activity of the Alkaloids from Ancistrocladus
tectorius Against the Larvae of Spodoptera litura
WANG Jun1， YAN Chao2， CAI Caihong1， FENG Gang2， ZHANG Jing2 *， DAI Haofu1 *
1 Institute of Tropical Bioscience and Biotechnology， Chinese Academy of Tropical Agricultural Sciences/Key Laboratory
of Biology and Genetic Resources of Tropical Crops， Ministry of Agriculture / Hainan Key Laboratory for Research
and Development of Natural Products from Li Folk Medicine， Haikou， Hainan 571101， China
2 Environment and Plant Protection Institute， Chinese Academy of Tropical Agricultural Sciences， Haikou， Hainan 571101， China
Abstract The insecticidal activity of the total alkaloids and its 3 compounds[（1）： Ancistrocline，（2）： 5′-O-demethylhamatintine，（3）： N-methylphylline]of Ancistrocladus tectorius against the larvae of Spodoptera litura were discussed for the development of new type of botanical pesticides. The toxicity of the total alkaloids to the 3rd instar larvae of S. litura was determined by insect-dip method. The toxicity of 3 compounds to the larvae of S. litura was tested by diet-incorporation. The results indicated that the total alkaloids of A. tectorius had strong toxic effect on the 3rd instar larvae and the LC50 values of 3 d and 5 d was 2.61 mg/mL and 0.91 mg/mL respectively. The growth of newly hatched instar larvae of S. litura was also inhibited by the three compounds. The compound（2）had the strongest inhibition effects with pupation rate 23.33% and eclosion rate 13.33%， which was significantly lower than those of control（pupation rate 90.00%， eclosion rate 86.67%）.
Key words Ancistrocladus tectorius； Alkaloids； Spodoptera litura； Insecticidal activity； Growth and development inhibition
doi 10.3969/j.issn.1000-2561.2015.09.017
斜紋夜蛾（Spodoptera litura Fabricius）屬鱗翅目（Lepidoptera）、夜蛾科（Noctuidae），是世界重要的農業害蟲之一。以幼蟲取食寄主植物的果實、花、葉等，寄主植物多達99科290多種[1]。在我國淮河以北間歇性發生為害，淮河以南常年發生為害。在大田栽培作物中主要為害棉花、大豆、煙草、甘薯、花生等[2]；而在蔬菜中則以十字花科蔬菜和水生蔬菜受害最為嚴重。近年來，斜紋夜蛾在各地大發生的頻率明顯提高，且呈上升趨勢[3-5]。由于化學藥劑的施用造成環境污染和害蟲耐藥性的問題，新型天然、高效、安全的物質被用來開發殺蟲劑已成為一種必然趨勢。